CCL4 antibody

Catalog No. ABIN465381

The Rabbit Polyclonal anti-CCL4 antibody (ABIN465381) specifically detects CCL4 in WB, ELISA and Neut. The antibody is reactive with Mouse samples.

Quick Overview for CCL4 antibody (ABIN465381)

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL4 Antibody

Specificity: Mouse MIP-1beta

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine MIP-1beta.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (8 - 10 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Murine MIP-1beta (100 ng/mL), a concentration of 8-10 μg/mL of this antibody is required. ELISA: To detect Murine MIP-1beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine MIP-1beta (LS-C104473) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine MIP-1beta. Western Blot: To detect Murine MIP-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine MIP-1beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Alternative Name: CCL4 / SYCA4

Background: Name/Gene ID: CCL4
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL4, ACT2, Act-2, AT744.1, C-C motif chemokine 4, Chemokine (C-C motif) ligand 4, CC chemokine ligand 4, HC21, LAG1, MIP1B, MIP1B1, LAG-1, SCYA2, Secreted protein G-26, SIS-gamma, MIP-1-beta, MIP-1-beta(1-69), Small-inducible cytokine A4, T-cell activation protein 2, G-26, Lymphocyte-activation gene 1, PAT 744, Protein H400, SCYA4

Gene ID: 6351

UniProt: P13236