DEFB4A antibody

Catalog No. ABIN465401

The Goat Polyclonal anti-DEFB4A antibody is suitable to detect DEFB4A in samples from Human. It has been validated for ELISA and WB.

Quick Overview for DEFB4A antibody (ABIN465401)

Target: DEFB4A (DEFB4) (Defensin, beta 4A (DEFB4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DEFB4A antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-DEFB4A Antibody

Specificity: Human BD-2

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human BD-2.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hBD-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BD-2 (LS-C104585) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBD-2. Western Blot: To detect hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for DEFB4A

Target: DEFB4A (DEFB4) (Defensin, beta 4A (DEFB4))

Alternative Name: DEFB4A / DEFB2

Background: Name/Gene ID: DEFB4A

Synonyms: DEFB4A, Beta-defensin 2, Beta-defensin 4A, DEFB-2, DEFB102, Defensin, beta 2, Defensin, beta 4, Defensin, beta 4A, DEFB4, DEFB2, HBD-2, Skin-antimicrobial peptide 1, BD-2, SAP1

Gene ID: 1673