CCL20 antibody

Catalog No. ABIN465458

This Rabbit Polyclonal antibody specifically detects CCL20 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CCL20 antibody (ABIN465458)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CCL20 Antibody

Specificity: Human MIP-3alpha

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MIP-3alpha.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human colon/rectum adenocarcinoma tissue. The recommended concentration is 0.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect Human MIP-3alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-3alpha (LS-C104612) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-3alpha. Western Blot: To detect HumanMIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: CCL20 / MIP-3-Alpha

Background: Name/Gene ID: CCL20
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL20, Beta chemokine exodus-1, Beta-chemokine exodus-1, CKb4, Exodus-1, MIP-3a, MIP-3-alpha, LARC, ST38, SCYA20, C-C motif chemokine 20, CC chemokine LARC, Mip-3alpha, MIP3A, Small-inducible cytokine A20

Gene ID: 6364

UniProt: P78556

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection