CCL20 antibody (Biotin)

Catalog No. ABIN465465

The Biotin-conjugated Rabbit Polyclonal anti-CCL20 antibody (ABIN465465) specifically detects CCL20 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for CCL20 antibody (Biotin) (ABIN465465)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL20 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CCL20 Antibody (Biotin)

Specificity: Human MIP-3alpha

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MIP-3alpha.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human MIP-3alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-3alpha. To detect Human MIP-3alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MIP-3alpha (LS-C104608) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-3alpha. Western Blot: To detect Human MIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: CCL20 / MIP-3-Alpha

Background: Name/Gene ID: CCL20
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL20, Beta chemokine exodus-1, Beta-chemokine exodus-1, CKb4, Exodus-1, MIP-3a, MIP-3-alpha, LARC, ST38, SCYA20, C-C motif chemokine 20, CC chemokine LARC, Mip-3alpha, MIP3A, Small-inducible cytokine A20

Gene ID: 6364

UniProt: P78556

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection