CCL5 antibody

Catalog No. ABIN465616

This Rabbit Polyclonal antibody specifically detects CCL5 in WB, ELISA and Neut. It exhibits reactivity toward Mouse.

Quick Overview for CCL5 antibody (ABIN465616)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL5 Antibody

Specificity: Mouse RANTES

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine RANTES.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (6 - 8 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained colchicine injected mouse brain (somatosensory cortex) tissue. The primary antibody was incubated at 0.5 μg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mRANTES (100.00 ng/mL), a concentration of 0.8-1.2 μg/mL of this antibody is required. ELISA: To detect mRANTES by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RANTES (LS-C104424) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mRANTES. Western Blot: To detect mRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRANTES is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 100 μL sterile ddH20

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: CCL5 / RANTES

Background: Name/Gene ID: CCL5
Family: Intercrine

Synonyms: CCL5, Chemokine (C-C motif) ligand 5, D17S136E, EoCP, T-cell specific protein p288, SCYA5, SISd, T cell-specific protein P228, Beta-chemokine RANTES, C-C motif chemokine 5, RANTES, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, TCP228

Gene ID: 6352

UniProt: P13501

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration