CCL5 antibody

Catalog No. ABIN465680

The Rabbit Polyclonal anti-CCL5 antibody is suitable to detect CCL5 in samples from Human. It has been validated for WB, ELISA and Neut.

Quick Overview for CCL5 antibody (ABIN465680)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL5 Antibody

Specificity: Human RANTES

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human RANTES.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, Neut (0.4 - 0.6 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hRANTES (100 ng/mL), a concentration of 0.4-0.6 μg/mL of this antibody is required. ELISA: To detect hRANTES by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human RANTES (LS-C104720) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hRANTES. Western Blot: To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: CCL5 / RANTES

Background: Name/Gene ID: CCL5
Family: Intercrine

Synonyms: CCL5, Chemokine (C-C motif) ligand 5, D17S136E, EoCP, T-cell specific protein p288, SCYA5, SISd, T cell-specific protein P228, Beta-chemokine RANTES, C-C motif chemokine 5, RANTES, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, TCP228

Gene ID: 6352

UniProt: P13501

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration