RANKL antibody
Quick Overview for RANKL antibody (ABIN465772)
Target
See all RANKL (TNFSF11) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Specificity
- Mouse sRANKL
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Purification
- Immunoaffinity purified
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Immunogen
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E.coli derived recombinant Murine sRANKL.
Type of Immunogen: Cell extract
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anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 221-300) antibody
TNFSF11 Reactivity: Human, Mouse, Rat, Rabbit WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 74-308) antibodyTNFSF11 Reactivity: Human, Monkey WB, ELISA, IHC, FACS, ICC Host: Mouse Monoclonal 8A7B9 unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 10-90) antibodyTNFSF11 Reactivity: Human, Mouse WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 210-317) antibodyTNFSF11 Reactivity: Human, Mouse, Rat, Rabbit WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (C-Term) antibodyTNFSF11 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 92-263) antibodyTNFSF11 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 106-240) antibodyTNFSF11 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (C-Term) antibodyTNFSF11 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 1-30) antibodyTNFSF11 Reactivity: Human WB, ELISA, IHC, FACS Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 222-316) antibodyTNFSF11 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 3F7 unconjugated
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Application Notes
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Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of msRANKL (50 ng/mL), a concentration of 05 μg/mL of this antibody is required. ELISA: To detect msRANKL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine sRANKL (LS-C104817) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant msRANKL. Western Blot: To detect msRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant msRANKL is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
Comment
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Target Species of Antibody: Mouse
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Sterile water
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS, pH 7.2
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Handling Advice
- Avoid freeze/thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
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Alternative Name
- TNFSF11 / RANKL / TRANCE
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Background
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Name/Gene ID: TNFSF11
Family: TNF
Synonyms: TNFSF11, CD254, CD254 antigen, HRANKL2, OPTB2, TRANCE, ODF, OPGL, SOdf, Osteoprotegerin ligand, RANKL -
Gene ID
- 8600
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UniProt
- O14788
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Pathways
- NF-kappaB Signaling
Target
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