Ccl12 antibody

Catalog No. ABIN465845

The Rabbit Polyclonal anti-Ccl12 antibody is suitable to detect Ccl12 in samples from Mouse. It has been validated for ELISA, WB and Neut.

Quick Overview for Ccl12 antibody (ABIN465845)

Target: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ccl12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Neutralization (Neut)

Product Details anti-Ccl12 Antibody

Specificity: Mouse MCP-5

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine MCP-5.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (2 - 3 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mMCP-5 (1000 ng/mL), a concentration of 2-3 μg/mL of this antibody is required. ELISA: To detect mMCP-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine MCP-5 (LS-C104806) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mMCP-5. Western Blot: To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Ccl12

Target: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Alternative Name: CCL12 / MCP5

Background: Name/Gene ID: Ccl12

Synonyms: Ccl12, MCP-5, MCP5, Scya12

Gene ID: 20293

UniProt: Q62401

Pathways: Cellular Response to Molecule of Bacterial Origin