MYDGF antibody

Catalog No. ABIN465899

The Rabbit Polyclonal anti-MYDGF antibody is suitable to detect MYDGF in samples from Mouse. It has been validated for ELISA and WB.

Quick Overview for MYDGF antibody (ABIN465899)

Target: MYDGF (D17Wsu104e) (DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYDGF antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-MYDGF Antibody

Specificity: Mouse SF-20

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine SF-20.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect mSF-20 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine SF-20 (LS-C104568) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mSF-20. Western Blot: To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for MYDGF

Target: MYDGF (D17Wsu104e) (DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e))

Alternative Name: MYDGF / SF20

Background: Name/Gene ID: MYDGF

Synonyms: MYDGF, C19orf10, IL-27, Interleukin 25, Interleukin-25, EUROIMAGE1875335, IL27w, R33729_1, SF20, IL-25, UPF0556 protein C19orf10

Gene ID: 56005

UniProt: Q969H8