IL28A antibody (Biotin)

Catalog No. ABIN465904

The Biotin-conjugated Rabbit Polyclonal anti-IL28A antibody (ABIN465904) specifically detects IL28A in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for IL28A antibody (Biotin) (ABIN465904)

Target: IL28A (Interleukin 28A (Interferon, lambda 2) (IL28A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL28A antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IL28A Antibody (Biotin)

Specificity: Human IFN-lambda2

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IFN-lambda2.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect Human IFN-lambda2 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IFN-lambda2. To detect Human IFN-lambda2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IFN-lambda2 (LS-B7476) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IFN-lambda2. Western Blot: To detect Human IFN-lambda2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-lambda2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL28A

Target: IL28A (Interleukin 28A (Interferon, lambda 2) (IL28A))

Alternative Name: IL28A

Background: Name/Gene ID: IFNL2

Synonyms: IFNL2, Interferon, lambda 2, IL-28A, IL28A

Gene ID: 282616

UniProt: Q8IZJ0