Elongation Factor 1 alpha antibody

Catalog No. ABIN472648

This Mouse Monoclonal antibody specifically detects Elongation Factor 1 alpha in IP and WB. It exhibits reactivity toward Trypanosoma brucei.

Quick Overview for Elongation Factor 1 alpha antibody (ABIN472648)

Target: Elongation Factor 1 alpha

Reactivity: Trypanosoma brucei

Host: Mouse

Clonality: Monoclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Specificity: Recognizes EF-1a (Elongation factor-1a) at 53kD. 38kD protein occasionally detected in A431 cell lysate. Species cross-reactivity: Trypanosomes, yeast and mammals.

Purification: Protein G purified

Immunogen: Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography.

Type of Immunogen: Protein extract

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IP (0.5 - 2 μg/mL), WB

Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using an IgG (HRP) Gt xMo secondary antibody and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitated EF-1 a from an A431 RIPA cell lysate.

Comment:

Target Species of Antibody: Trypanosoma brucei

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details

Target: Elongation Factor 1 alpha