Methylated Lysine antibody (HRP)

Catalog No. ABIN479105

The Rabbit Polyclonal anti-Methylated Lysine antibody is suitable to detect Methylated Lysine. It has been validated for WB and ELISA.

Quick Overview for Methylated Lysine antibody (HRP) (ABIN479105)

Target: Methylated Lysine

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA

Product Details

Specificity: Detects proteins containing methylated lysine residues in SDS-PAGE immunoblots. Species cross-reactivity: Multi-species.

Purification: Immunoaffinity purified

Immunogen: Methylated lysine, KLH conjugated.

Type of Immunogen: Hapten

Application Details

Application Notes: Approved: ELISA (0.2 - 0.5 μg/mL), WB (0.2 - 0.5 μg/mL)

Usage: Suitable for use in Western Blot and ELISA. Western Blot: 0.2-0.5 μg/mL was sufficient for detection of the methylated histone by Western Blot analysis using melanoma cells in TBSt. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 50 % glycerol.

Handling Advice: Aviod frequent freeze thaws.

Storage: -20 °C

Storage Comment: Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

Target Details

Target: Methylated Lysine

Alternative Name: Methyl-Lysine

Target Type: Chemical