Macrophage/Monocyte antibody

Catalog No. ABIN479363

The Mouse Monoclonal anti-Macrophage/Monocyte antibody is suitable to detect Macrophage/Monocyte in samples from Mouse, Human and Rat. It has been validated for FACS, IHC (fro), IHC, IHC (p), IP, RIA and WB.

Quick Overview for Macrophage/Monocyte antibody (ABIN479363)

Target: Macrophage/Monocyte

Reactivity: Mouse, Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)

Product Details

Specificity: Recognizes a single chain glycoprotein of 90-100kD that is expressed on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen has many characteristics in common with macrosialin in the mouse and CD68 in the human.

Purification: Purified

Immunogen: Rat Spleen cells.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:100 - 1:200), IHC, IHC-Fr, IHC-P, IP, RIA, WB

Usage: Suitable for use in Immunohistochemistry ( frozen and paraffin embedded sections), Flow Cytometry, Immunoprecipitation, Western Blot and Radioimmunoassay. Immunohistochemistry on frozen sections at 1:50-1:300. Paraffin embedded tissue (Bouin fixation): 1:50-1:300 (requires pretreatment with trypsin or pronase). Flow cytometry: 1:100-1:200. Use 10 μL of this working dilution to label 1 x10⁶.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details

Target: Macrophage/Monocyte

Alternative Name: Monocytes, Macrophages