Macrophage/Monocyte antibody

Catalog No. ABIN481685

The Rat Monoclonal anti-Macrophage/Monocyte antibody is suitable to detect Macrophage/Monocyte in samples from Mouse. It has been validated for IHC and IHC (p).

Quick Overview for Macrophage/Monocyte antibody (ABIN481685)

Target: Macrophage/Monocyte

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ER-HR3

Product Details

Specificity: The antigen is found on mouse monocytes and a subset of macrophages. The epitope has not been further characterized. Antigen distribution: Isolated cells: The antigen is found on up to 70 % of circulating monocytes, all other leukocytes are ER-HR 3 negative. It is also found on a subpopulation (about 30 %) of bone marrow cells, mainly consisting of myeloid cells. Tissue sections: In the adult mouse, the antigen is found on distinct subpopulations of resident tissue macrophages in various organs. It is found on a subpopulation of the splenic red pulp macrophages, in the mesenteric lymphoid paracortex, interfollicular areas of Peyer's patches and bone marrow. Epidermal Langerhans cells also express the antigen, whereas macrophages in the connective tissue of the dermis and the gastrointestinal tract only scarcely express the ER-HR 3 related antigen. In the kidney, ER-HR 3 positive macrophages belong to the type 2 interstitial cells in the outer medulla which are negative with BM 8. Distinct ER-HR 3 positive macrophage subpopulations are found in various embryological organs where haematopoietic islands occur, and where they are closely associated with erythrocyte precursor cells. Specificity: Mouse: subpopulation of mature macrophages. Other species: not tested

Purification: Affinity purified

Immunogen: Adherent bone marrow cells

Isotype: IgG2c

Application Details

Application Notes: Approved: IHC, IHC-P (1:100)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 10 mg/mL BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details

Target: Macrophage/Monocyte

Alternative Name: Monocytes, Macrophages