MHC Class II antibody

Catalog No. ABIN481691

The Rat Monoclonal anti-MHC Class II antibody is suitable to detect MHC Class II in samples from Mouse. It has been validated for IHC and IHC (fro).

Quick Overview for MHC Class II antibody (ABIN481691)

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MHC Class II antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ER-TR2

Product Details anti-MHC Class II Antibody

Specificity: MHC Class II antigens are heterodimers consisting of one a- chain (31-34  kDa) and one beta-chain (26-29  kDa). The epitope has not been further characterized. Antigen distribution Isolated cells: The antigen is found on dendritic cells, B-cells and macrophages. The level of antigen detected by ER-TR1, ER-TR2 and ER-TR3 differs from strain to strain (see table below). Tissue Sections: The antigen is found on B-cells, interdigitating cells and macrophages in peripheral lymphoid organs but is absent from T-cells. It is also expressed as a fine reticular pattern on stromal thymic cells of the cortex and as a confluent pattern on stromal thymic cells of the medulla. Specificity: Mouse: cells expressing MHC class II antigens Other species: negative on human, others not tested.

Purification: Affinity purified

Immunogen: murine thymic reticulum

Application Details

Application Notes: Approved: IHC, IHC-Fr (1:200)

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 5 mg/mL BSA, 0.05 % Kathon

Preservative: Kathon CG

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Alternative Name: MHC Class II