Macrophages antibody

Catalog No. ABIN481695

The Mouse Monoclonal anti-Macrophages antibody (Clone RM-1) (ABIN481695) specifically detects Macrophages in IHC (p) and IHC. The antibody is reactive with Rat samples.

Quick Overview for Macrophages antibody (ABIN481695)

Target: Macrophages

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Clone: RM-1

Product Details

Specificity: The antigen is localized on the surface of cells. It is stable to 2 % periodate-lysine-paraformaldehyde fixation. The epitope has not been further characterized. Antigen distribution: Isolated cells: Positive on monocytes in peripheral blood. Positive on 20-30 % of peritoneal cells, increasing to 30-40 % after thioglycolate stimulation. Tissue sections: The antigen is found on a subpopulation of most tissue fixed macrophages. It is quite predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells. Specificity: Rat: monocytes, subpopulation of macrophages, interdigitating cells. Other species: not tested

Purification: Affinity purified

Immunogen: Peritoneal macrophages

Application Details

Application Notes: Approved: IHC, IHC-P (1:100)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 10 mg/mL BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details

Target: Macrophages