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Antibodies
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anti-Histone H1 (Acetyl-Lys25) antibody
Histone H1 (Acetyl-Lys25) antibody
| Antigen |
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| Synonyms |
H1c, H1.2, H1f2, His1a, H1var1, MGC107646, 0610008C09Rik, LOC692942, HISTONE H1, HON4 |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Immunofluorescence (IF) (36), Western Blotting (WB) (27), Flow Cytometry (FACS) (18), ELISA (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (15), Immunohistochemistry (IHC) (9), Immunoprecipitation (IP) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunocytochemistry (ICC) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN486132 |
| Quantity |
50µg (1 mg/ml) |
| Price |
150.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Histone H1 (Acetyl-Lys25)
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Gene ID
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132243 (NCBI Gene Symbol: H1FOO)
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Swiss-Prot
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Q8IZA3
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Immunogen
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The antiserum was produced against synthesized peptide derived from human Histone H1 around the acetylated site of Lys25.
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Format
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Liquid
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Synonyms: H1, H1 histone family member 5, H1.5, H15, H1F5, HIST1H1B, histone 1, histone H1.5, histone H1a
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Specificity
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Histone H1 (Acetyl-Lys25) Antibody detects endogenous levels of total Histone H1 protein only when acetylated at lysine25.
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Molecular Weight
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21 kDa
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Comments
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Unigene-Number: Hs.97358
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Application Notes
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WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
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Concentration
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1 mg/ml
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Purity
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Greater 95%
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
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Buffer
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Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C/1 year
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Restrictions
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For Research Use only
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Alternatives for antigen "Histone H1", type "Antibodies"
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Hosts
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Rabbit (27), Mouse (18), Chicken (4), Sheep (4)
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Reactivities
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Human (42), Cow (Bovine) (21), Mouse (Murine) (21), Rat (Rattus) (17), Dog (Canine) (16), Horse (Equine) (16), Rabbit (16), Pig (Porcine) (15)
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Applications
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Immunofluorescence (IF) (36), Western Blotting (WB) (27), Flow Cytometry (FACS) (18), ELISA (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (15), Immunohistochemistry (IHC) (9), Immunoprecipitation (IP) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunocytochemistry (ICC) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pThr17 (1)
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