NK2 Homeobox 5 (NKX2-5) antibody

Antigen: NK2 Homeobox 5 (NKX2-5)

Synonyms: NKX2-5, NKX2.5, MGC137749, Nkx2.5, Nkx2-5, csx, csx1, nkx2e, nkx2.5, tinman, Nkx-2.5, XNkx2-5, XNkx-2.5

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Rabbit

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN486853

Additional Information

Alternative name: NKX2-5

Gene ID: Nkx2-5

UniProt: P52952

Immunogen: The immunogen for anti-Nkx2-5 antibody: synthetic peptide directed towards the n terminal of human Nkx2-5

Sequence: ELGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKK ELCALQKAVE

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-Nkx2-5 antibody concentration is 1 mg/ml.

Description: Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1211 U34962.1 1-1211 1212-1585 BC025711.1 1143-1516

Characteristics: Antigen Size: 324 amino acids

Molecular Weight: 35kDa

Application Details

Application Notes: WB Suggested Anti-Nkx2-5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: MCF7 cell lysate.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-Nkx2-5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: MCF7 cell lysate

Publications

Product: Nagel, Meyer, Quentmeier et al.: "MEF2C is activated by multiple mechanisms in a subset of T-acute lymphoblastic leukemia cell lines." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 22, Issue 3, pp. 600-7, 2008 (PubMed).