AIRE antibody (Middle Region)

Catalog No. ABIN486887

The Rabbit Polyclonal anti-AIRE antibody is suitable to detect AIRE in samples from Human, Rat, Dog, Horse and Guinea Pig. It has been validated for WB.

Quick Overview for AIRE antibody (Middle Region) (ABIN486887)

Target: AIRE (Autoimmune Regulator (AIRE))

Reactivity: Human, Rat, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIRE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AIRE Antibody

Binding Specificity: Middle Region

Sequence: GGSKKCIQVG GEFYTPSKFE DSGSGKNKAR SSSGPKPLVR AKGAQGAAPG

Predicted Reactivity: Dog: 77%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against AIRE. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AIRE

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 545 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: AIRE

Background: The function of the protein encoded by the AIRE gene is not well defined. However it contains zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).
Alias Symbols: AIRE1, APECED, APS1, APSI, PGA1
Protein Interaction Partner: TUBB, RAVER1, TUBA1C, TMPRSS13, CCDC87, FAT1, GIGYF2, SRRM2, TUBB4B, EIF4EBP3, UBB, HSP90AB1, HSPA8, HSPA5, HSPA1L, HSPA1A, ATP4A, HIST3H3, HDAC2, HDAC1, DAXX, AIRE, UBAC1, HIST1H3A, HIST2H2BE, HSPA4, SRSF1, SMC1A, XPO1, TOP2A, SSRP1, SNRPD3, SNRPB, SRSF3
Protein Size: 545

Molecular Weight: 58 kDa

Gene ID: 326

NCBI Accession: NM_000383, NP_000374

UniProt: O43918

Pathways: Chromatin Binding