TRPA1 antibody (Middle Region)

Catalog No. ABIN487135

This Rabbit Polyclonal antibody specifically detects TRPA1 in WB, IHC and IF. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Dog, Horse, Cow, Rabbit and Saccharomyces cerevisiae. It has been mentioned in 4+ publications

Quick Overview for TRPA1 antibody (Middle Region) (ABIN487135)

Target: TRPA1 (Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1))

Reactivity: Human, Mouse, Rat, Guinea Pig, Dog, Horse, Cow, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TRPA1 Antibody

Binding Specificity: Middle Region

Sequence: KCTDRLDEDG NTALHFAARE GHAKAVALLL SHNADIVLNK QQASFLHLAL

Predicted Reactivity: Cow: 93%, Dog: 90%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Yeast: 100%

Characteristics: This is a rabbit polyclonal antibody against TRPA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRPA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1119 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TRPA1 antibody (ABIN487135)

Trevisan, Benemei, Materazzi, De Logu, De Siena, Fusi, Fortes Rossato, Coppi, Marone, Ferreira, Geppetti, Nassini: "TRPA1 mediates trigeminal neuropathic pain in mice downstream of monocytes/macrophages and oxidative stress." in: Brain : a journal of neurology, Vol. 139, Issue Pt 5, pp. 1361-77, (2016) (PubMed).

Kun, Perkecz, Knie, Sétáló, Tornóczki, Pintér, Bán: "TRPA1 receptor is upregulated in human oral lichen planus." in: Oral diseases, Vol. 23, Issue 2, pp. 189-198, (2016) (PubMed).

Pierce, Ryals, Wang, Christianson: "Vaginal hypersensitivity and hypothalamic-pituitary-adrenal axis dysfunction as a result of neonatal maternal separation in female mice." in: Neuroscience, Vol. 263, pp. 216-30, (2014) (PubMed).

Nassini, Pedretti, Moretto, Fusi, Carnini, Facchinetti, Viscomi, Pisano, Stokesberry, Brunmark, Svitacheva, McGarvey, Patacchini, Damholt, Geppetti, Materazzi: "Transient receptor potential ankyrin 1 channel localized to non-neuronal airway cells promotes non-neurogenic inflammation." in: PLoS ONE, Vol. 7, Issue 8, pp. e42454, (2012) (PubMed).

Target Details for TRPA1

Target: TRPA1 (Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1))

Alternative Name: TRPA1

Background: The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined, however, studies indicate the function may involve a role in sig
Alias Symbols: ANKTM1
Protein Interaction Partner: UBC, CYLD,
Protein Size: 1119

Molecular Weight: 127 kDa

Gene ID: 8989

NCBI Accession: NM_007332, NP_015628

UniProt: O75762