Phosphotyrosine antibody (phosphorylated)

Catalog No. ABIN487507

This Mouse Monoclonal antibody specifically detects Phosphotyrosine in WB, IP, IF and IHC (fro).

Quick Overview for Phosphotyrosine antibody (phosphorylated) (ABIN487507)

Target: Phosphotyrosine

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: G104

Product Details anti-Phosphotyrosine Antibody

Binding Specificity: phosphorylated

Purification: Protein G Chromatography.

Immunogen: Phosphotyrosine, alanine and glyceine in a 1: 1: 1 ratio polymerized in the presence ofKeyhole Limpet Hemocyanin with 1-ethyl-3-(3?-dimentrylaminopropyl) carbodiimide.

Isotype: IgG1

Application Details

Application Notes: Western blot: 1/1000 (1,2).1 μg/mL of this antibody was sufficient for detection of phosphorylated tyrosine residues in10 μg of Rat tissue lysate by colorimetric Immunoblot analysis using Goat anti-Rat IgG-HRPas the secondary antibody. Immunoprecipitation (9). Immunofluorescence (2). Immunohistochemitry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (3). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8).