Bromelain antibody

Catalog No. ABIN488556

The Rabbit Polyclonal anti-Bromelain antibody (ABIN488556) specifically detects Bromelain in ELISA, WB, IF and IHC. The antibody is reactive with Pineapple samples.

Quick Overview for Bromelain antibody (ABIN488556)

Target: Bromelain

Reactivity: Pineapple

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details

Purpose: Anti-Bromelain

Cross-Reactivity (Details): Ananas comosus
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Ananas comosus

Purification: Purified IgG

Immunogen: native bromelain isolated and purified from Ananas comosus

Isotype: IgG

Application Details

Application Notes: 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)

Comment:

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 1 mL of sterile destilled water

Buffer: Purified IgG in PBS.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: Bromelain

Background:

Background: Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.

Additional information: Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition

Molecular Weight: 39 kDa