SHANK2 antibody (PDZ Domain, SH3 Domain)

Catalog No. ABIN492458

The Mouse Monoclonal anti-SHANK2 antibody is suitable to detect SHANK2 in samples from Human and Rat. It has been validated for WB, IP, IF and IHC (fro).

Quick Overview for SHANK2 antibody (PDZ Domain, SH3 Domain) (ABIN492458)

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHANK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: S23b-6

Product Details anti-SHANK2 Antibody

Binding Specificity: AA 84-309, PDZ Domain, SH3 Domain

Specificity: Detects ~160 kDa protein. No cross-reactivity against Shank1 or Shank3.

Characteristics: Synonyms: Cortactin-binding protein 1, CORTBP1, PROSAP1, SPANK-3, SPANK3, KIAA1022, SH3 andmultiple ankyrin repeat domains protein 2, Proline-rich synapse-associated protein 1

Purification: Protein G Chromatography.

Immunogen: Fusion protein amino acids 84-309 (SH3/PDZ domains) of Rat Shank2 (SH3 and multipleAnkyrin repeat domains protein 2 (accession number Q9QX74).

Isotype: IgG1

Application Details

Application Notes: Western blot: 1 μg/mL1 μg/mL was sufficient for detection of Shank2 in 10 μg of rat brain lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for SHANK2

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Alternative Name: SHANK2

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (3). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina (4). Other recent studies suggest that the disruption of glutamate receptors at the Shankpostsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer's disease (5).Synonyms: CORTBP1, Cortactin-binding protein 1, KIAA1022, PROSAP1, Proline-rich synapse-associated protein 1, SH3 and multiple ankyrin repeat domains protein 2, SPANK-3, SPANK3

Gene ID: 171093

UniProt: Q9QX74