Lamin B2 (LMNB2) (AA 221-470) antibody

Antigen: Lamin B2 (LMNB2)

Synonyms: LMN2, LAMB2, MGC2721, lamin, RGD1563803, lmnb2-a, LMNB2, lmn2, lamb2, Lamin-L(II), LOC100136040

Epitope: AA 221-470

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN497934

Additional Information

Characteristics: Synonyms: Lamin-B2, LMNB2, LMNB-2, LMN2, LMNB, Nuclear Envelope Marker

Alternative name: Lamin-B2 (LMNB2)

Gene ID: 84823

Swiss-Prot: Q03252

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within aminoacids 221 and 470 of Lamin B2

Cross-Reactivity: Human

Description: An important part of the cell nucleus is formed by nuclear lamina. Nuclear lamins form anetwork of filaments at the nucleoplasmic site of the nuclear membrane. Two mainsubtypes of nuclear lamins can be distinguished, i. e. A-type lamins and B-type lamins. TheA-type lamins comprise a set of three proteins arising from the same gene by alternativesplicing, i. e. Lamin A, Lamin C and lamin Adel10, while the B-type lamins include twoproteins arising from two distinct genes, i. e. Lamin B1 and Lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteinsin that they are fibrous molecules that have an aminoterminal globular head, a central rodof a-helices and a carboxyterminal globular domain. Many biochemical and molecularfeatures of lamins have been studied, but their functions remain still largelyundetermined. One of the functions ascribed to the lamina is the maintenance of thestructural integrity of the nucleus. Besides interactions with the nuclear membrane andother intermediate filaments, lamins interact with the nuclear chromatin. Eukaryoticchromatin is organised into loops, which are attached to the nuclear matrix. Thisorganisation is thought to contribute to compaction of the chromatin and regulation ofgene expression. Lamins, as part of the nuclear matrix, may be involved in these processessince chromatin binding sites have been detected in both A- and B-type Lamins.

Specificity: Recognizes Lamin B2 (221-470). Cellular Localization: Nucleus inner membrane, Lipid-anchor, Nucleoplasmic sideSpecies Reactivity: Tested: Human. Add. Information: Predicted Molecular Weight: 68 kDa

Application Details

Application Notes: Western blotting: 1/500-1/3000. Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol and 0.01% Thimerosal aspreservative.

Storage: Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only