GOPC antibody (C-Term)

Catalog No. ABIN497983

This Rabbit Polyclonal antibody specifically detects GOPC in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GOPC antibody (C-Term) (ABIN497983)

Target: GOPC (Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GOPC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-GOPC Antibody

Binding Specificity: C-Term

Specificity: Recognizes PIST (C-term).

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat

Purification: Immunoaffinity Chromatography

Immunogen: PIST antibody was raised against a 16 amino acid peptide from near the carboxy terminus of Human PIST

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: PIST antibody can be used for the detection of PIST at 1-2 μg/mL. Positive Control: Rat Colon Cell Lysate. Immunohistochemistry on paraffin sections. Positive Control: Rat Colon Cell Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.

Target Details for GOPC

Target: GOPC (Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC))

Alternative Name: GOPC / PIST

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (4-6). Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1 (6).Synonyms: CAL, CFTR-associated ligand, FIG, Fused in glioblastoma, Golgi-associated PDZ and coiled-coil motif-containing protein, PDZ protein interacting specifically with TC10

Gene ID: 57120

NCBI Accession: NP_001017408

UniProt: Q9HD26

Pathways: Maintenance of Protein Location, Asymmetric Protein Localization