PEX2 antibody (Internal Region)

Catalog No. ABIN498038

The Goat Polyclonal anti-PEX2 antibody is suitable to detect PEX2 in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB and EIA.

Quick Overview for PEX2 antibody (Internal Region) (ABIN498038)

Target: PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PEX2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PEX2 Antibody

Binding Specificity: Internal Region

Sequence: C-NATVGQSVLN IKYKN

Specificity: Recognizes PEX2/PXMP3.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the Internal region of the protein sequence according to NP_000309.1.

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/128000. Western blot: 1-3 μg/mL. Approx 35 kDa band observed in lysates of cell line HeLa(calculated MW of 34.5 kDa according to NP_000309.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PEX2

Target: PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))

Alternative Name: Peroxin 2 / PEX2 / RNF72

Background: This gene encodes an integral peroxisomal membrane protein required for peroxisome biogenesis. The protein is thought to be involved in peroxisomal matrix protein import. Mutations in this gene result in one form of Zellweger syndrome and infantile Refsum disease. Alternative splicing results in multiple transcript variants encoding the same protein.Synonyms: 35 kDa peroxisomal membrane protein, PAF-1, PAF1, PMP3, PMP35, PXMP3, Peroxin-2, Peroxisomal membrane protein 3, Peroxisome assembly factor 1, Peroxisome biogenesis factor 2, RING finger protein 72

Gene ID: 5828

NCBI Accession: NP_000309

UniProt: P28328

Pathways: Monocarboxylic Acid Catabolic Process