Acid Phosphatase, Prostate (ACPP) (Internal Region) antibody

Details for Product No. ABIN498060
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Antigen
Synonyms ACPP, CD73, Nt5, 4921514H13Rik, AIRP, CN-IB, 5'-NT, ACP-3, ACP3, PAP, A030005E02Rik, FRAP, Lap, Ppal, Acpp11, RNACPP11, pap, acp-3, acp3, acpt, AMAP2, CENTB3, DDEF2, PAG3, Pap-alpha, SHAG1
Epitope
Internal Region
(7), (6), (6), (2), (2), (2), (2), (2), (2), (2), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(112), (25), (21), (13), (4), (2), (1), (1), (1)
Host
Goat
(52), (44), (16), (6), (2), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(47), (44), (36), (27), (14), (11), (10), (8), (7), (6), (3), (2), (2), (2), (2), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN498060
368.50 $
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Immunogen Peptide from the internal region of Human ACPP according to NP_001090.2. Genename: ACPP
Sequence YGIHKQKEKS RLQ
Specificity This antibody is expected to recognize both isoforms (NP_001090.2, NP_001127666.1).
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name ACPP / Prostatic Acid Phosphatase
Background Prostatic acid phosphatase is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase.Synonyms: ACP3, PAP, PSAP, Prostate acid phosphatase
Molecular Weight 44.6kDa (NP_001090.2)
Gene ID 55
NCBI Accession NP_001090
UniProt P15309
Application Notes Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.3-1 μg/mL. Detects a band of Approx 50 kDa in Human Prostate lysates. The observed molecular weightcorresponds to earlier findings in literature with different antibodies (Patel et al. , ClinChem.1986 Oct,32(10): 1832-5. PMID: 3530530).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Quintero, Araujo, Pulkka et al.: "Prostatic acid phosphatase is not a prostate specific target." in: Cancer research, Vol. 67, Issue 14, pp. 6549-54, 2007 (PubMed).

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