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ACPP antibody (Acid Phosphatase, Prostate) (Internal Region)

Details for Product anti-ACPP Antibody No. ABIN498060, Supplier: Login to see
Antigen
  • ACPP
  • CD73
  • Nt5
  • 4921514H13Rik
  • AIRP
  • CN-IB
  • 5'-NT
  • ACP-3
  • ACP3
  • PAP
  • A030005E02Rik
  • FRAP
  • Lap
  • Ppal
  • Acpp11
  • RNACPP11
  • pap
  • acp-3
  • acp3
  • acpt
  • AMAP2
  • CENTB3
  • DDEF2
  • PAG3
  • Pap-alpha
  • SHAG1
Epitope
Internal Region
31
12
11
11
7
4
3
3
3
3
2
2
2
2
2
2
2
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
172
27
15
4
2
1
1
1
1
1
Host
Goat
85
54
38
7
2
1
Clonality
Polyclonal
Conjugate
This ACPP antibody is un-conjugated
11
8
8
3
3
3
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
91
90
59
44
12
11
9
8
5
3
2
2
2
2
2
2
1
Supplier
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Immunogen Peptide from the internal region of Human ACPP according to NP_001090.2. Genename: ACPP
Sequence YGIHKQKEKS RLQ
Specificity This antibody is expected to recognize both isoforms (NP_001090.2, NP_001127666.1).
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name ACPP / Prostatic Acid Phosphatase (ACPP Antibody Abstract)
Background Prostatic acid phosphatase is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase.Synonyms: ACP3, PAP, PSAP, Prostate acid phosphatase
Molecular Weight 44.6kDa (NP_001090.2)
Gene ID 55
NCBI Accession NP_001090
UniProt P15309
Pathways
Application Notes Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.3-1 μg/mL. Detects a band of Approx 50 kDa in Human Prostate lysates. The observed molecular weightcorresponds to earlier findings in literature with different antibodies (Patel et al. , ClinChem.1986 Oct,32(10): 1832-5. PMID: 3530530).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ACPP antibody (Acid Phosphatase, Prostate) (Internal Region) (ABIN498060) AP20162PU-N ACPP Antibody staining of paraffin embedded Human Prostate at 3.8 µg/ml. ...
Western Blotting (WB) image for anti-ACPP antibody (Acid Phosphatase, Prostate) (Internal Region) (ABIN498060) AP20162PU-N ACPP Antibody staining of Human Prostate lysate at 0.3 µg/ml (35µg protei...
Background publications Quintero, Araujo, Pulkka et al.: "Prostatic acid phosphatase is not a prostate specific target." in: Cancer research, Vol. 67, Issue 14, pp. 6549-54, 2007 (PubMed).