PBX1 antibody (Internal Region)

Catalog No. ABIN498074

The Goat Polyclonal anti-PBX1 antibody is suitable to detect PBX1 in samples from Human, Mouse, Rat and Dog. It has been validated for WB and EIA.

Quick Overview for PBX1 antibody (Internal Region) (ABIN498074)

Target: PBX1 (Pre-B-Cell Leukemia Homeobox Protein 1 (PBX1))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PBX1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PBX1 Antibody

Binding Specificity: Internal Region

Sequence: C-ATNVSAHGSQ ANSPS

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of the protein sequence according to NP_002576.1. Genename: PBX1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/32000. Western blot: 0.5-1.5 μg/mL. Detects a band of Approx 45 kDa in lysates of cell line K562.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse, Rat, Canine (Dog). Add. Information: Calculated Molecular Weight: 46.5 kDa according to NP_002576.1

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PBX1

Target: PBX1 (Pre-B-Cell Leukemia Homeobox Protein 1 (PBX1))

Alternative Name: PBX1

Background: This nonactivating protein which could be a repressor, binds the sequence 5'-ATCAATCAA-3'. It is converted into a potent transcriptional activator by the (1,19) translocation. PBX1 May have a role in steroidogenesis and subsequently sexual development and differentiation, and is expressed in all tissues except in cells of the B and T lineage. PBX1 is involved in a form of pre-B-cell acute lymphoblastic leukemia (B-ALL) (25 % of human pediatric cases) through a chromosomal translocation t(1,19)(q23,p13.3) which involves PBX1 and E2A genes. E2A-PBX1 transforms cells by constitutively activating transcription of genes regulated by PBX1 or by other members of the PBX protein family. PBX1 belongs to the TALE/PBX homeobox family, and contains 1 homeobox domain.Synonyms: Homeobox protein PBX1, Homeobox protein PRL, Pre-B-cell leukemia transcription factor 1

Gene ID: 5087

NCBI Accession: NP_002576

UniProt: P40424