Dystroglycan antibody (Internal Region)

Catalog No. ABIN498078

The Goat Polyclonal anti-Dystroglycan antibody (ABIN498078) specifically detects Dystroglycan in WB and EIA. The antibody is reactive with Human, Mouse, Rat, Cow and Dog samples.

Quick Overview for Dystroglycan antibody (Internal Region) (ABIN498078)

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Dystroglycan antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Dystroglycan Antibody

Binding Specificity: Internal Region

Sequence: C-HVGKHEYFMH ATDK

Specificity: This antibody is expected to recognize both the precursor and the mature Alpha-Dystroglycan, but not the mature Beta-Dystroglycan.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of the protein sequence according to NP_004384.3. Genename: DAG1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/128000. Western blot: 0.2-0.6 μg/mL. Detects a band of Approx 100 kDa in Human Skeletal Muscle lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Dystroglycan

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Alternative Name: Dystroglycan

Background: Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha Dystroglycan and a 43 kDa transmembrane protein, beta Dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin.Synonyms: DAG1, Dystrophin-associated glycoprotein 1

Gene ID: 1605

NCBI Accession: NP_004384

UniProt: Q14118

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus