CDC16 antibody

Catalog No. ABIN498111

The Rabbit Polyclonal anti-CDC16 antibody (ABIN498111) specifically detects CDC16 in WB, IF and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for CDC16 antibody (ABIN498111)

Target: CDC16 (Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC16 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDC16 Antibody

Specificity: This antibody detects endogenous levels of Cdc16 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % Sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for CDC16

Target: CDC16 (Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16))

Alternative Name: CDC16 / APC6

Background: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cell division cycle protein 16 homolog (CDC16) is a component of the APC (anaphase-promoting complex), which is composed of eight proteins and functions as a protein ubiquitin ligase. The APC complex is a cyclin degradation system that governs exit from mitosis. Each component protein of the APC complex is highly conserved among eukaryotic organisms.Synonyms: ANAPC6, Anaphase-promoting complex subunit 6, CDC16Hs, Cell division cycle protein 16 homolog, Cyclosome subunit 6

Molecular Weight: approx. 72 kDa

Gene ID: 8881

NCBI Accession: NP_001072113

UniProt: Q13042

Pathways: Regulation of Actin Filament Polymerization