MAF antibody

Catalog No. ABIN498275

The Rabbit Polyclonal anti-MAF antibody is suitable to detect MAF in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for MAF antibody (ABIN498275)

Target: MAF (V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian) (MAF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAF Antibody

Specificity: This antibody detects endogenous levels of c-Maf protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for MAF

Target: MAF (V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian) (MAF))

Alternative Name: MAF

Background: Members of the Maf family of basic region/leucine zipper (bZIP) transcription factors affect transcription in either a positive or negative fashion, depending on their particular protein partner and the context of the target promoter. c-Maf (Maf-2) and the closely related family members Neural retina leucine zipper (Nrl), L-Maf, and Krml1/MafB (Maf-1) all bind to T-MARE sites and have been implicated in a wide variety of developmental and physiologic roles. The three small Maf family proteins (MafF, MafG, and MafK) are components of NF-E2 that function as heterodimers with the large tissuerestricted subunit of NF-E2 called p45, and they are implicated in the transcriptional regulation of many erythroid-specific genes. MafB is expressed in a wide variety of tissues and encodes a protein containing a typical bZip motif in its carboxy-terminal region. As a transcriptional activator, MafB plays a pivotal role in regulating lineage-specific gene expression during hematopoiesis by repressing Ets-1-mediated transcription of key erythroidspecific genes in myeloid cells.Synonyms: Proto-oncogene c-maf, Transcription factor Maf, V-maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog

Molecular Weight: approx. 41 kDa

Gene ID: 4094

NCBI Accession: NP_001026974

UniProt: O75444