RORA antibody

Catalog No. ABIN498312

The Rabbit Polyclonal anti-RORA antibody (ABIN498312) specifically detects RORA in WB, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for RORA antibody (ABIN498312)

Target: RORA (RAR-Related Orphan Receptor A (RORA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RORA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RORA Antibody

Specificity: This antibody detects endogenous levels of RORα protein. (region surrounding Gly41)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemitry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for RORA

Target: RORA (RAR-Related Orphan Receptor A (RORA))

Alternative Name: ROR-alpha / RORA

Background: Retinoid related orphan receptor alpha (ROR alpha) is a NR1 Thyroid Hormone Like Receptor. To date, ROR alph,a bet,a and gamma subtypes have been discovered. ROR alpha has been shown to affect the development of the central nervous system. Deletion of ROR alpha in mice causes severe impairment in the differentiation of cerebellar Purkinje neurons and results in the staggerer phenotype. ROR alpha has been suggested as a potential target in the treatment of chronic inflammatory diseases, including atherosclerosis and rheumatoid arthritis.Synonyms: NR1F1, Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, Nuclear receptor subfamily 1 group F member 1, RZRA, Retinoid-related orphan receptor-alpha

Molecular Weight: approx. 60 kDa

Gene ID: 6095

NCBI Accession: NP_002934

UniProt: P35398

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha