ELAVL2 antibody

Catalog No. ABIN498332

The Rabbit Polyclonal anti-ELAVL2 antibody is suitable to detect ELAVL2 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for ELAVL2 antibody (ABIN498332)

Target: ELAVL2 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 2 (Hu Antigen B) (ELAVL2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELAVL2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ELAVL2 Antibody

Specificity: This antibody detects endogenous levels of Hel-N1 protein. (region surrounding Ser27)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for ELAVL2

Target: ELAVL2 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 2 (Hu Antigen B) (ELAVL2))

Alternative Name: ELAVL2 / HUB

Background: The Elav-like genes encode for a family of RNA-binding proteins. Elav, a Drosophila protein and the first described member, is expressed immediately after neuroblastic differentiation into neurons and is necessary for neuronal differentiation and maintenance. Several mammalian Elav-like proteins, designated HuC, HuD and Hel-N1, are also expressed in postmitotic neurons. An additional mammalian homolog, HuR, which is also designated HuA, is ubiquitously expressed and is also overexpressed in a wide variety of tumors. Characteristically, these homologs all contain three RNA recognition motifs (RRM), and they specifically bind to AU-rich elements (ARE) in the 3'-untranslated region of mRNAs transcripts. ARE sites target mRNA for rapid degradation and thereby regulate the expression levels of genes involved in cell growth and differentiation.Synonyms: ELAV-like neuronal protein 1, ELAV-like protein 2, Hel-N1, Hu-antigen B, Nervous system-specific RNA-binding protein Hel-N1

Molecular Weight: approx. 38 kDa

Gene ID: 1993

NCBI Accession: NP_004423

UniProt: Q12926