FOXC1 antibody

Catalog No. ABIN498333

The Rabbit Polyclonal anti-FOXC1 antibody (ABIN498333) specifically detects FOXC1 in WB, IF and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for FOXC1 antibody (ABIN498333)

Target: FOXC1 (Forkhead Box C1 (FOXC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXC1 Antibody

Specificity: This antibody detects endogenous levels of FoxC1/2 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Also reacts to: FOXC2

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FOXC1

Target: FOXC1 (Forkhead Box C1 (FOXC1))

Alternative Name: FOXC1 / FKHL7 / FREAC3

Background: FOXC2 is a member of forkhead/winged helix transcription factor family whose members serve as key regulators in embryogenesis and cell differentiation. FOXC2 functions as a key regulator of adipocyte metabolism by increasing the sensitivity of the β-adrenergic-cAMP-protein kinase A (PKA) signaling pathway through alteration of adipocyte PKA holoenzyme composition. Increased FOXC2 levels, induced by high fat diet, seem to counteract most of the symptoms associated with obesity. FOXC2 expression is also associated with the early stage of chondrogenic differentiation both in vivo and in vitro. FOXC2 haploinsufficiency results in Lymphedema-distichiasis (LD), an autosomal dominant disorder that classically presents as lymphedema of the limbs and double rows of eyelashes (distichiasis). Mutant mice null for FOXC2 show defects in axial and cranial skeletogenesis, suggesting a requirement of FOXC2 for skeletal tissue development.Synonyms: FREAC-3, Forkhead box protein C1, Forkhead-related protein FKHL7, Forkhead-related transcription factor 3

Molecular Weight: approx. 57 kDa

Gene ID: 2296

NCBI Accession: NP_001444

UniProt: Q12948

Pathways: Chromatin Binding, Glycosaminoglycan Metabolic Process