SET/TAF-I antibody

Catalog No. ABIN498343

This Rabbit Polyclonal antibody specifically detects SET/TAF-I in WB and IHC (p). It exhibits reactivity toward Human and Rat.

Quick Overview for SET/TAF-I antibody (ABIN498343)

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SET/TAF-I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SET/TAF-I Antibody

Specificity: This antibody detects endogenous levels of TAF I p48 protein. (region surrounding Arg318)

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SET/TAF-I

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Alternative Name: SET / I2PP2A

Background: For gene transcription governed by RNA polymerase I, the human transcription factor SL1 (mouse TIF-IB) directs the assembly of initiation complexes at the prompter. Like TFIID, which directs transcription by RNA polymerase II, SL1/TIF-IB contains the TATA-binding protein (TBP) and a set of TBP-associated factors (TAFs). The three TAF I subunits, hTAF I p110, hTAF I p63 and hTAF p48 (or mouse TAF I p95, TAF I p68 and TAF I p48) are all integral components of SL1/TIF-IB. The mutually exclusive binding of either TAF I or TAF II subunits to TBP is believed to direct the formation of promoter and RNA polymerase-specific complexes.Synonyms: I-2PP2A, IGAAD, Inhibitor of granzyme A-activated DNase, PHAPII, Phosphatase 2A inhibitor I2PP2A, TAF-I, Template-activating factor I

Molecular Weight: approx. 53 kDa

Gene ID: 6418

NCBI Accession: NP_001116293

UniProt: H0UI37

Pathways: Apoptosis