CCAR2 antibody

Catalog No. ABIN498347

The Rabbit Polyclonal anti-CCAR2 antibody is suitable to detect CCAR2 in samples from Human. It has been validated for WB.

Quick Overview for CCAR2 antibody (ABIN498347)

Target: CCAR2 (Cell Cycle and Apoptosis Regulator 2 (CCAR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCAR2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CCAR2 Antibody

Specificity: This antibody detects endogenous levels of KIAA1967 / DBC-1 protein. (region surrounding Gln455)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % pure SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM Sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CCAR2

Target: CCAR2 (Cell Cycle and Apoptosis Regulator 2 (CCAR2))

Alternative Name: KIAA1967

Background: KIAA1967 / DBC-1 (deleted in breast cancer gene 1 protein), also known as p30 DBC protein, is one of the genes located within the region of chromosome 8 (8p21-8p23) that is homozygously deleted in some breast cancers. DBC-1 contains a nuclear localization signal, an N-terminal leucine zipper, an EF hand and a C-terminal coiled-coil region. DBC-1 is closely related to DIS but lacks the SAP domain. During death signaling mediated by TNFα, endogenous DBC-1 undergoes caspase-dependent processing to generate DBC-1 p120 and p66, both of which include the C-terminus of the protein. Both DBC-1 p120 and p66 relocate to the cytoplasm. Overexpression of the DBC-1 p120 form results in mitochondrial clustering and matrix condensation and increases the sensitivity of cells to TNFalpha-mediated apoptosis. In addition, DBC-1 directly interacts with unliganded ERalpha, stabilizing its expression and therefore collaborating to suppress apoptosis and promote hormone-independent cell growth.Synonyms: DBC-1, DBC.1, DBC1, Deleted in breast cancer gene 1 protein, KIAA1967, p30 DBC

Molecular Weight: approx. 102.0 kDa

Gene ID: 57805

NCBI Accession: NP_066997

UniProt: Q8N163