PRKG2 antibody

Catalog No. ABIN498430

This Rabbit Polyclonal antibody specifically detects PRKG2 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PRKG2 antibody (ABIN498430)

Target: PRKG2 (Protein Kinase, CGMP-Dependent, Type II (PRKG2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKG2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRKG2 Antibody

Specificity: This antibody detects endogenous levels of cGKii protein. (region surrounding Asp412)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PRKG2

Target: PRKG2 (Protein Kinase, CGMP-Dependent, Type II (PRKG2))

Alternative Name: CGK2

Background: CGKII (cGMP-dependent protein kinase type II) is a major receptor of intracellular cGMP, and mediates a plethora of physiological responses. cGKII has a predicted molecular weight of 86 kD,a and contains a conserved leucine zipper motif at the amino terminus. It is expressed in small intestine, colon, prostate, and human brain tissues, and the cGKII gene maps to chromosome 4q13.1-q21.1. cGKII has been shown to regulate the ion transport system in the intestine. Myristoylation of the penultimate glycine in cGKII appears to be essential for directing cGKII to the membrane, since cGKII is devoid of any hydrophobic transmembrane domains. The translocation of cGKII from the cytosol to the membrane allows it to function properly in regulating intestinal ion transport.Synonyms: PRKG2, PRKGR2, Type II cGMP-dependent protein kinase, cGKII, cGMP-dependent protein kinase 2

Molecular Weight: approx. 90 kDa

Gene ID: 5593

NCBI Accession: NP_006250

UniProt: Q13237