A Kinase (PRKA) Anchor Protein 1 (AKAP1) antibody

Antigen: A Kinase (PRKA) Anchor Protein 1 (AKAP1)

Synonyms: AKAP, PRKA1, AKAP84, AKAP121, AKAP149, D-AKAP1, MGC1807, SAKAP84, Akap, C76494, C81186, DAKAP1, S-AKAP84, AKAP1, akap1, fa08b04, MGC162884, wu:fa08b04, Akap84

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN498433

Additional Information

Characteristics: Synonyms: AKAP149, PRKA1, AKAP-1, A kinase anchor protein 1 mitochondrial, Protein kinaseA-anchoring protein 1, A-kinase anchor protein 149 kDa, Dual specificityA-kinase-anchoring protein 1, Spermatid A-kinase anchor protein 84

Alternative name: AKAP1

Gene ID: 8165

Swiss-Prot: Q92667

Cross-Reactivity: Human

Description: The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range ofsubstrates. Specificity of PKA signaling is thought to be mediated by thecompartmentalization of the kinase to specific sites within the cell. To maintain thisspecific localization, the R subunit (RII) of PKA interacts with specific RII-anchoringproteins. This family of proteins has been designated A-kinase anchoring proteins (AKAP). Members of this family, including MAP2 (microtubule-associated protein 2), neuronallyexpressed AKAP 79 and AKAP 150, and the DNA binding AKAP 95, display differential tissuespecificity and localization. Evidence suggests that AKAP 79 and AKAP 150 are bothcapable of anchoring PKA to postsynaptic densities (PSD), which are a network of proteinslocated on the internal surfaces of excitatory synapses.

Specificity: AKAP 149 pAb detects endogenous levels of AKAP 149 protein. Species: Human. Others not tested.

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1,0 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling

Restrictions: For Research Use only