Laminin, alpha 4 (LAMa4) antibody

Antigen: Laminin, alpha 4 (LAMa4)

Synonyms: LAMA3, LAMA4*-1, DKFZp686D23145, RGD1560062, si:dkey-119m7.3, si:busm1-189a20.2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN498447

Additional Information

Characteristics: Synonyms: LAMA4, Laminin subunit alpha-4

Alternative name: Laminin alpha 4

Gene ID: 3910

Swiss-Prot: Q16363

Cross-Reactivity: Human, Mouse (Murine)

Description: Laminins are essential and abundant structural non-collagenous glycoproteins localizingto basement membranes. Basement membranes (cellassociated extracellular matrices(ECMs)) are polymers of laminins with stabilizing type IV collagen networks, nidogen, andseveral proteoglycans. Basement membranes are found under epithelial layers, around theendothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration,gene expression, and tissue architecture. Each laminin is a heterotrimer of α, β, and γchain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins canself-assemble, bind to other matrix macromolecules, and have unique and shared cellinteractions mediated by integrins, dystroglycan, and cognate laminin receptors. Thehuman laminin α-4 gene maps to chromosome 6q21 and is expressed in adult heart, lung,ovary, small and large intestines, liver, and placenta.

Specificity: This antibody detects endogenous levels of Laminin alpha-4 protein. Species: Human and Mouse. Others not tested. Add. Information: Molecular Weight: 203 kDa

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM Sodium Azide as preservative,approx. pH 7.2

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only