DIDO1 antibody

Catalog No. ABIN498449

The Rabbit Polyclonal anti-DIDO1 antibody is suitable to detect DIDO1 in samples from Human and Mouse. It has been validated for WB, IF and IHC (p).

Quick Overview for DIDO1 antibody (ABIN498449)

Target: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIDO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DIDO1 Antibody

Specificity: This antibody detects endogenous levels of DIDO1 / Dio-1 protein. (region surrounding Ser186)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DIDO1

Target: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Alternative Name: DIDO1

Background: DIDO1 / Dio-1 (Death Inducer-Obliterator-1) is a putative transcription factor that contains two zinc finger motifs. Dio-1 translocates to the nucleus, and activates apoptosis during limb development. Programmed cell death, a highly regulated form of apoptosis, plays an important role in determining the amount of tissue, the shape, and the definition of each digit during limb development. Dio-1 expression is upregulated when an apoptotic signal is detected, and subsequently apoptosis is induced. This process is similar to the expression of NFκB and NGF in response to external signals. Dio-1 expression is suppressed by caspase inhibitors and Bcl-2 expression. This supports the theory that Dio-1 functions in the onset of programmed cell death.Synonyms: C20orf158, DATF1, Death-inducer obliterator 1, KIAA0333

Molecular Weight: approx. 244 kDa

Gene ID: 11083

NCBI Accession: NP_071388

UniProt: Q9BTC0

Pathways: Hormone Activity