Interferon gamma antibody

Catalog No. ABIN498507

The Rabbit Polyclonal anti-Interferon gamma antibody (ABIN498507) specifically detects Interferon gamma in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Interferon gamma antibody (ABIN498507)

Target: Interferon gamma (IFNG)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Interferon gamma Antibody

Specificity: This antibody detects endogenous levels of IFN-gamma protein. (region surrounding Gln69)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG / Interferon gamma

Background: Interferon (IFN)-gamma is an antiviral and antiparasitic agent produced by CD4+/ CD8+ lymphocytes and natural killer cells that undergo activation by antigens, mitogens or alloantigens. IFN-gamma production modulates T cell growth and differentiation and inhibits the growth of B cells. Synthesis of IFN-gamma is inducible by IL-2, FGF and EGF. The active form of IFN-gamma is a homodimer with each subunit containing six helices. The dimeric structure of human IFN-gamma is stabilized by non-covalent interactions through the interface of the helices. IFN-gamma tranlsated precursor is 166 amino acids, including the 23 amino acid secretory sequence. 20 kDa and 25 kDa forms exist due to variable glycosylation, 40-60 kDa forms are observable under non-denaturing conditions as dimers and tetramers.Synonyms: IFN-gamma, gamma IFN

Molecular Weight: approx. 22 kDa

Gene ID: 3458

NCBI Accession: NP_000610

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy