PAR1 antibody

Catalog No. ABIN498947

The Rabbit Polyclonal anti-PAR1 antibody is suitable to detect PAR1 in samples from Human. It has been validated for WB.

Quick Overview for PAR1 antibody (ABIN498947)

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAR1 Antibody

Specificity: This antibody detects endogenous levels of Thrombin R protein. (region surrounding Ser42)

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Alternative Name: Thrombin Receptor / F2R

Background: Thrombin is a serine protease that is involved in platelet aggregation and blood coagulation. It is cleaved from its precursor, prothrombin, and converts fibrinogen to fibrin in the final step of the clotting cascade. Thrombin mediates its regulatory effects by activating cell surface receptors. These receptors, including thrombin receptor (also designated PAR-1, for protease-activated receptor-1), PAR-2 and PAR-3 are members of the G-protein coupled receptor family, and share a similiar gene structure. Thrombin cleaves its receptor, releasing a 41 amino acid peptide which acts as a platelet agonist. Upon this activation by thrombin, the thrombin receptors trigger an increase in cytosolic Ca2+ concentration. Unactivated thrombin receptor cycles between the cell surface and an intracellular pool, while activated receptor internalizes rapidly and is degraded in the lysosomes. The human thrombin receptor is also known to be regulated by Sp1 and Sp3 transcription factors.Synonyms: CF2R, Coagulation factor II receptor, PAR-1, PAR1, Proteinase-activated receptor 1

Molecular Weight: approx. 48 kDa

Gene ID: 2149

NCBI Accession: NP_001983

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus