Add to Basket
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Activating Transcription Factor 1 (AFT1) antibody

Antigen

Activating Transcription Factor 1 (AFT1)
Synonyms TREB36, EWS-ATF1, FUS/ATF-1, Atf-1, zgc:64099, wu:fc92c07, treb36, MGC89098, ews-atf1, fus/atf-1, ATF1, MGC143110
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Catalog no. ABIN498985
Quantity 100 µg  (1,0 mg/ml)  (Variants)
Price 340.00 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Characteristics Synonyms: ATF-1, Activating transcription factor 1, Protein TREB36, Cyclic AMP-dependent transcriptionfactor ATF-1, cAMP-dependent transcription factor ATF-1
Alternative name ATF1
Gene ID 466
Swiss-Prot P18846
Cross-Reactivity Human, Mouse (Murine)
Description Eukaryotic gene transcription is regulated by sequencespecific transcription factors thatbind modular cis acting promoter and enhancer elements. The ATF/ CREB transcriptionfactor family binds the palindromic cAMP response element (CRE) octanucleotideTGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1,ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, butshare a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to playa key role in the induction of NOX1, this protein binds the cAMP response element (CRE)and mediates PKAinduced stimulation of CRE-reporter genes. ATF-2 forms homodimers andheterodimers with c-Jun to initiate CREdependent transcription. Phosphorylation of ATF-2at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life ofATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2Band H4 specifically in vitro.
Specificity ATF-1 pAb detects endogenous levels of ATF-1 protein.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1,0 mg/ml
Purification Aff - Purified
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Activating Transcription Factor 1 (AFT1)", type "Antibodies"
Hosts Rabbit (28), Mouse (6), Goat (3), Sheep (1)
Reactivities Human (35), Rat (Rattus) (24), Mouse (Murine) (23), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1), Guinea Pig (1), Horse (Equine) (1), Pig (Porcine) (1), Rabbit (1), Yeast (Saccharomyces Cerevisiae) (1)
Applications Immunofluorescence (IF) (20), Western Blotting (WB) (20), Flow Cytometry (FACS) (18), ELISA (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Enzyme Immunoassay (EIA) (4), Immunoprecipitation (IP) (4), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)