DOCK1 antibody

Catalog No. ABIN499057

This Rabbit Polyclonal antibody specifically detects DOCK1 in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for DOCK1 antibody (ABIN499057)

Target: DOCK1 (Dedicator of Cytokinesis 1 (DOCK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOCK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DOCK1 Antibody

Specificity: This antibody detects endogenous levels of DOCK 180 protein. (region surrounding Lys1692)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DOCK1

Target: DOCK1 (Dedicator of Cytokinesis 1 (DOCK1))

Alternative Name: DOCK1 / DOCK180

Background: The v-Crk oncogene product shares homologous amino acid sequences, designated Src homology region 2 (SH2) and SH3, with many molecules involved in signal transduction. The v-Crk cellular homolog, c-Crk, is a member of a newly emerging class of genes including Nck and GRB2/ASH which encode proteins that consist primarily of SH2 and SH3 domains. Two distinct human c-Crk cDNAs, designated Crk I and Crk II, have been identified and shown to represent alternative splice products of c-Crk. The major translational product of c-Crk I has been identified as a 28 kDa variably expressed protein, while c-Crk II encodes a widely expressed 40 kDa protein and a more variably expressed 42 kDa protein. The major c-Crk transforming activity appears associated with c-Crk I p28 expression. DOCK 180, a 180 kDa protein downstream of Crk, has been identified as a major Crk-associated protein. When DOCK 180 is recruited to the plasma membrane from a cytoplasmic reservoir, presumably by Crk, changes in cellular morphology and spindle formation occur, suggesting DOCK 180 to be a Crk effector molecule.Synonyms: 180 kDa protein downstream of CRK, Dedicator of cytokinesis protein 1

Molecular Weight: approx. 215 kDa

Gene ID: 1793

NCBI Accession: NP_001371

UniProt: Q14185

Pathways: Signaling of Hepatocyte Growth Factor Receptor