CD3EAP antibody

Catalog No. ABIN499063

This Rabbit Polyclonal antibody specifically detects CD3EAP in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD3EAP antibody (ABIN499063)

Target: CD3EAP (CD3e Molecule, epsilon Associated Protein (CD3EAP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3EAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD3EAP Antibody

Specificity: This antibody detects endogenous levels of CD3EAP protein. (region surrounding Glu474)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for CD3EAP

Target: CD3EAP (CD3e Molecule, epsilon Associated Protein (CD3EAP))

Alternative Name: CD3EAP

Background: CD3EAP, also known as ASE1, CAST or PAF49, is a 510 amino acid protein that localizes to the nucleus and belongs to the eukaryotic RPA34 RNA polymerase subunit family. Existing as two alternatively spliced isoforms, CD3EAP functions as a component of the Pol I complex, specifically exhibiting DNA-dependent RNA polymerase activity and effectively catalyzing the the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. Isoform 2 of CD3EAP may be a component of the T cell receptor (TCR) complex and is subject to tyrosine phosphorylation in response to TCR stimulation. Both isoforms of CD3EAP are subject to DNA damage-dependent phosphorylation, probably by ATM or ATR.Synonyms: A34.5, ASE1, Anti-sense to ERCC-1 protein, CAST, CD3-epsilon-associated protein, DNA-directed RNA polymerase I subunit RPA34, PAF49, RNA polymerase I-associated factor PAF49

Molecular Weight: approx. 55 kDa

Gene ID: 10849

NCBI Accession: NP_036231

UniProt: O15446