ACO2 antibody (Internal Region)

Catalog No. ABIN499115

The Goat Polyclonal anti-ACO2 antibody is suitable to detect ACO2 in samples from Human, Rat, Mouse, Pig, Dog and Cow. It has been validated for WB, IHC (p) and EIA.

Quick Overview for ACO2 antibody (Internal Region) (ABIN499115)

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Reactivity: Human, Rat, Mouse, Pig, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This ACO2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ACO2 Antibody

Binding Specificity: AA 541-555, Internal Region

Sequence: C-QDTYQHPPKD SSGQH

Specificity: This antibody recognizes Aconitase 2 (541-555).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine, Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_001089.1. Genename: ACO2

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/6000. Western blot: 0.01-0.03 μg/mL. Approx 90 kDa band observed in Human Heart lysates(calculated MW of 85.0 kDa according to NP_001089.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ACO2

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Alternative Name: ACO2

Background: Aconitase 2 (ACO2) is an essential iron-dependent metabolic enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle.Synonyms: Aconitase 2, Aconitate hydratase mitochondrial

Gene ID: 50

NCBI Accession: NP_001089

UniProt: Q99798