Phospholamban antibody (N-Term)

Catalog No. ABIN499141

Product Details anti-Phospholamban Antibody

Target: Phospholamban (PLN)

Binding Specificity: N-Term

Reactivity: Human, Rat, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Phospholamban antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: KVQYLTRSAI RR-C

Specificity: Recognizes Phospholamban / PLN.

Cross-Reactivity (Details): Species reactivity (expected):Pig, Bovine.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_002658.1. Genename: PLN

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.01-0.03 μg/mL. This antibody detects a band of approx. 13 kDa band in Human andRat Heart lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Phospholamban

Target: Phospholamban (PLN)

Alternative Name: Phospholamban (PLN Products)

Synonyms: LOC100226261 antibody, plb antibody, cmd1p antibody, CMD1P antibody, CMH18 antibody, PLB antibody, Plb antibody, Plm antibody, phospholamban antibody, PLN antibody, pln antibody, Pln antibody

Background: Phospholamban (PLB/PLN) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains cAMP-dependent protein kinase phosphorylation sites and is highly basic and readily cleaved by proteases, whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. PLB is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.Synonyms: Cardiac phospholamban, PLB, PLN

Gene ID: 5350

NCBI Accession: NP_002658

UniProt: P26678

Pathways: Negative Regulation of Transporter Activity