CNN3 antibody (C-Term)

Catalog No. ABIN499177

This Goat Polyclonal antibody specifically detects CNN3 in WB. It exhibits reactivity toward Human and Cow.

Quick Overview for CNN3 antibody (C-Term) (ABIN499177)

Target: CNN3 (Calponin 3, Acidic (CNN3))

Reactivity: Human, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This CNN3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CNN3 Antibody

Binding Specificity: C-Term

Sequence: C-HGEYQDDYPR DYQYS

Specificity: This antibody detects Calponin-3 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):CowSpecies reactivity (tested):Human

Purification: Affinity chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_001830.1

Application Details

Application Notes: Peptide ELISA: 1: 64000 (limit dilution). Western blot: 0.2-0.6 μg/mL. Approx 37 kDa band observed in Human Duodenum, Heart andKidney lysates (calculated MW of 36.7 kDa according to NP_001830.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

Target Details for CNN3

Target: CNN3 (Calponin 3, Acidic (CNN3))

Alternative Name: Calponin-3

Background: Calponin is a thin filament associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Unlike basic calponin, the expression of acidic calponin is not restricted to smooth muscle, it can be detected in a number of different cells and tissues.Synonyms: CNN3, Calponin acidic isoform

Gene ID: 1266

NCBI Accession: NP_001830

UniProt: Q15417