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Details for Product No. ABIN499473

Peptidyl-tRNA Hydrolase 2 (PTRH2) (Middle Region) antibody

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Antigen
Synonyms BIT1, PTH2, Bit1, CGI-147, A230072I16Rik, RGD1306819, wu:fj09f02, zgc:109954
Epitope
»Alternatives Middle Region
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 3 references available
Catalog no. ABIN499473
Quantity 0.1 mg
Price
390.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Human Bit1 / Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2 (Intermediate Domain) Peptide (AP30153CP-N)
Isotype IgG
Specificity Bit1 antibody was raised against a 16 amino acid peptide from near the center of human Bit1.
Purification Affinity chromatography purified via peptide column
Alternative Name PTRH2 / BIT1
Background Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process (2). Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (3). It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE (2). Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (2).
Alternate names: CGI-147, PTH2, Peptidyl-tRNA hydrolase 2 mitochondrial
Gene ID 51651
NCBI Accession NP_057161
UniProt Q9Y3E5
Application Notes ELISA. Western Blot: Bit1 antibody can be used for the detection of Bit1 at 1 - 2 μg/ml. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide.
Preservative Sodium azide
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Chen, Courey: "Groucho/TLE family proteins and transcriptional repression." in: Gene, Vol. 249, Issue 1-2, pp. 1-16, 2000 (PubMed).

Jan, Matter, Pai et al.: "A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors." in: Cell, Vol. 116, Issue 5, pp. 751-62, 2004 (PubMed).

Martin, Vuori: "Regulation of Bcl-2 proteins during anoikis and amorphosis." in: Biochimica et biophysica acta, Vol. 1692, Issue 2-3, pp. 145-57, 2004 (PubMed).

Alternatives for antigen "Peptidyl-tRNA Hydrolase 2 (PTRH2)", type "Antibodies"
Hosts (50), (2)
Reactivities (50), (30), (23), (12), (12), (12), (1)
Applications (41), (17), (14), (9), (9), (7), (4), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (11), (6), (4), (2), (2), (2), (2), (1)
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