DAD1 antibody

Catalog No. ABIN499706

The Rabbit Polyclonal anti-DAD1 antibody has been validated for WB, IF and EIA. It is suitable to detect DAD1 in samples from Human and Mouse.

Quick Overview for DAD1 antibody (ABIN499706)

Target: DAD1 (Defender Against Cell Death 1 (DAD1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-DAD1 Antibody

Specificity: DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1.

Purification: Ion exchange chromatography

Immunogen: Murine DAD1 Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 0.5 to 2 μg/mL. Despite its predicted molecular weight, DAD1 migrates at approx. 22 kDa in SDS-PAGE. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for DAD1

Target: DAD1 (Defender Against Cell Death 1 (DAD1))

Alternative Name: DAD1

Background: Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death (1), a process important for normal organism development and tissue homeostasis (2). DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity (3,4). Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death (5,6). Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas (7), indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.Synonyms: DAD-1, OST2, defender against cell death 1, dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit DAD1, oligosaccharyl transferase subunit DAD1

Gene ID: 1603

UniProt: P61803